TY - JOUR T1 - Effects of exercise training and stem cell therapy on the left ventricle of infarcted rats JO - Revista Portuguesa de Cardiologia T2 - AU - de Freitas,Juliana S. AU - Neves,Clóvis A. AU - Del Carlo,Ricardo J. AU - Belfort,Felipe G. AU - Lavorato,Victor N. AU - Silame-Gomes,Luis H.L. AU - Ramos,Regiane M.S. AU - Cunha,Daise Q.N. da AU - Okano,Bárbara S. AU - Pereira,Vanessa G. AU - de Oliveira,Edilamar M. AU - Carneiro-Júnior,Miguel A. AU - Natali,Antônio J. SN - 08702551 M3 - 10.1016/j.repc.2019.02.013 DO - 10.1016/j.repc.2019.02.013 UR - https://www.revportcardiol.org/pt-effects-exercise-training-stem-cell-articulo-S0870255119305359 AB - Introduction and ObjectivesStem cell therapy and aerobic exercise are non-pharmacological therapies following myocardial infarction. The aim of this study was to test whether aerobic exercise training enhances the benefits of mesenchymal stem cell (MSC) therapy on remodeling of the extracellular matrix and fetal gene expression in the left ventricle of infarcted rats. MethodsMyocardial infarction was surgically induced in six-week old male Wistar rats. Animals were divided into four groups: sedentary control (SC) and sedentary and stem cell treated (SCMSC); exercised (EX) and exercised and stem cell treated (EXMSC). Bone marrow-derived MSCs were immediately transplanted via the tail vein (concentration: 1×106 cells). Exercise training (five days/week, 60 min/day; 60% of maximal running speed) started 24 hours after myocardial infarction and lasted for 12 weeks. ResultsExercise capacity was higher in exercised than in sedentary groups. Animals in the SCMSC, EX and EXMSC groups exhibited better cardiac function than those in SC. Collagen content was lower in the SCMSC, EX and EXMSC groups than in SC and skeletal α-actin expression was lower in EX and EXMSC than in SC. The α/β-MHC ratio was higher in EX and EXMSC than in SC. The combination of therapies further reduced collagen content in the remote region of the infarct (∼24%) and skeletal α-actin expression (∼30%). ConclusionAerobic exercise training appears to enhance the beneficial effects of stem cell therapy on remodeling of the extracellular matrix and fetal gene expression in the left ventricle of rats with moderate infarction. ER -